Mutagenically separated PCR (MS-PCR): a highly specific one step procedure for easy mutation detection

Mutagenically separated PCR (MS-PCR): a highly specific one step procedure for easy mutation detection.

With increasing knowledge about the causal role of genetic defects in clinical diseases the necessity is apparent to have procedures for rapid diagnosis of point mutations. We developed a PCR-based technique, whereby both normal and mutant alleles can be amplified in the same reaction tube, using different length allele-specific primers. Furthermore the allele-specific primers introduce additio...

Directed termination PCR: a one-step approach to mutation detection.

We describe a novel PCR-based method that allows the generation of nested termination fragments by integrating both selective DNA amplification and directed chain termination into a single PCR reaction. These termination fragments can be examined for sequence variation in either denaturing or non-denaturing polyacrylamide gels. This method provides a one-step and highly effective approach for t...

One-Step Reverse Transcription PCR (RT-PCR). Fatemeh Mehrpooyan*

Multiplex mutagenically separated PCR: diagnosis of beta-thalassemia and hemoglobin variants.

A rapid and simple method, termed multiplex mutagenically separated PCR (MS-PCR), was developed to detect several molecular defects in the hemoglobin gene in one PCR. This technique, in which different-size allele-specific primers were used, specifically amplified both normal and mutant alleles of the globin gene in the same reaction. Subsequent gel electrophoresis showed at least one of the tw...

PCR optimization: Improving of Human Cytomegalovirus (HCMV) PCR to Achieve a Highly Sensitive Detection Method

Polymerase chain reaction (PCR) is a rapid and simple technique with high sensitivity and specificity. Inthe recent years, PCR has been used for rapid detection of viral nucleic acids, such as Humancytomegalovirus (HCMV), whereas, PCR optimization is an important task to be done, especially beforeit’s diagnostic application. Annealing temperature, ion concentration (especially...